Application Note: Rapid Separation of Cholesterol by ELSD
UV & ELSD double trigger fractionation system Pure C-815! Highly productive cholesterol ELSD rapid fractionation.
Related Device: Fractionation Chromatography System Pure C-815 Cholesterol and its esters, which are components of biological membranes, are utilized as industrial raw materials in cosmetics, pharmaceuticals, and liquid crystals. When performing gradient fractionation purification for compounds that are difficult to detect with UV detectors, methods are selected using low wavelength UV detection such as 210 nm or ELSD (Evaporative Light Scattering Detector). ELSD is a fractionation purification method that overcomes the disadvantages of UV-triggered fractionation due to the absence of baseline drift and T0 peaks. By adopting ELSD & UV double triggering, it enables complementary fractionation purification using both ELSD and UV. 【Issues with Low Wavelength UV Triggered Fractionation】 ■ The baseline drifts due to the solvent's own absorption used in the mobile phase, making it difficult to detect sample peaks. ■ Baseline rises consistently during gradient steps, leading to the baseline being recognized as a peak, which significantly increases purification time and fraction volume. *For more details, please refer to the PDF document or feel free to contact us.
- Company：日本ビュッヒ
- Price：Other
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